Difference between revisions of "Part:BBa K4044001:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | Since the BphP1 nucleotide sequence is too large, the two parts of the sequences were obtained separately. Application of TIIS restriction enzymes allow to divide the BphP1 nucleotide sequence into the desired fragments and subsequently to join them. | ||
+ | |||
Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool. | Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool. | ||
Revision as of 09:42, 21 October 2021
BphP1 (E.coli optimized)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 246
Illegal BamHI site found at 1821 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504
Illegal NgoMIV site found at 402
Illegal NgoMIV site found at 1153
Illegal NgoMIV site found at 1650 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since the BphP1 nucleotide sequence is too large, the two parts of the sequences were obtained separately. Application of TIIS restriction enzymes allow to divide the BphP1 nucleotide sequence into the desired fragments and subsequently to join them.
Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.
Source
BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 and codon optimized for E. coli.
Source organism: Rhodopseudomonas palustris