Difference between revisions of "Part:BBa K4011004"
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<partinfo>BBa_K4011004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4011004 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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TnaA is an tryptophanase found in <i>E. coli</i>. It converts tryptophan (trp) and other related molecules, such as 6-Halogen-trp (6-X-trp) into indole or 6-X-indole. Its specific reaction formula is L-tryptophan + H₂O ⇌ indole + pyruvate + NH₃. Indole, play important roles in signaling in bacterial cells. FMO is a monooxygenase found in <i>Methylophaga aminisulfidivorans</i>. It adds a hydroxyl group onto numerous molecules, in our case adding a hydroxyl onto the third carbon on indole or 6-X-indole, allowing for spontaneous dimerization of 3-hydroxyl-indole or 3-hydroxyl-6-X-indole into indigo or tyrian purple dyes. They are fused together with the common rigid linker EAAAKEAAAK (Lee et al, 2021).
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Revision as of 09:37, 21 October 2021

TnaA-FL-FMO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1219
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TnaA is an tryptophanase found in E. coli. It converts tryptophan (trp) and other related molecules, such as 6-Halogen-trp (6-X-trp) into indole or 6-X-indole. Its specific reaction formula is L-tryptophan + H₂O ⇌ indole + pyruvate + NH₃. Indole, play important roles in signaling in bacterial cells. FMO is a monooxygenase found in Methylophaga aminisulfidivorans. It adds a hydroxyl group onto numerous molecules, in our case adding a hydroxyl onto the third carbon on indole or 6-X-indole, allowing for spontaneous dimerization of 3-hydroxyl-indole or 3-hydroxyl-6-X-indole into indigo or tyrian purple dyes. They are fused together with the common rigid linker EAAAKEAAAK (Lee et al, 2021).