Difference between revisions of "Part:BBa K3788002:Design"
 
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<p>After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.</p>
 
<p>After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.</p>
  
−
<p>This part is from the <b>BBa_K2938003 part</b>, the sequence was optimised for <i> E. coli </i>. The sequence codding for 6 histidin tag was added in 5' of the Part:BBa_K2938008 optimised </p>
+
<p>This part is from the <b>BBa_K2938003 part</b>, the sequence was optimised for <i> E. coli </i>. The sequence coding for 6 histidin tag was added in 5' of the Part:BBa_K2938008 optimised </p>
  
  

Latest revision as of 09:06, 21 October 2021


6His-P20


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.

This part is from the BBa_K2938003 part, the sequence was optimised for E. coli . The sequence coding for 6 histidin tag was added in 5' of the Part:BBa_K2938008 optimised


Source

References

Manasherob, R. et al. Effect of Accessory Proteins P19 and P20 on Cytolytic Activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. Curr. Microbiol. 43, 355–364 (2001).