Difference between revisions of "Part:BBa K3712017"
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<p><b>Figure 1.</b>SDS-PAGE of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP of purification. lane 1/2 is two-tube stream, lane 3 For Wild-type BLP-7-27 ELP, a thicker band appears near the 15kDa marker; lane 4/5/6 is a three-pipe stream that collects Optimized BLP-7-27 ELP, and a thicker band appears near the 15kDa marker.</p> | <p><b>Figure 1.</b>SDS-PAGE of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP of purification. lane 1/2 is two-tube stream, lane 3 For Wild-type BLP-7-27 ELP, a thicker band appears near the 15kDa marker; lane 4/5/6 is a three-pipe stream that collects Optimized BLP-7-27 ELP, and a thicker band appears near the 15kDa marker.</p> | ||
<p>After culturing, induction and sterilization, the results of nickel column affinity chromatography show that both of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP elution have single peaks at 80 mM with good symmetry, which preliminarily indicates that the target protein is purified. Then we take the BCA method to determine the concentration (Wild-type BLP-7-27 ELP 1.5mg/ml and Optimized BLP-7-27 ELP 1.2mg/ml) and SDS-PAGE to verify the existence of product.</p> | <p>After culturing, induction and sterilization, the results of nickel column affinity chromatography show that both of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP elution have single peaks at 80 mM with good symmetry, which preliminarily indicates that the target protein is purified. Then we take the BCA method to determine the concentration (Wild-type BLP-7-27 ELP 1.5mg/ml and Optimized BLP-7-27 ELP 1.2mg/ml) and SDS-PAGE to verify the existence of product.</p> | ||
Latest revision as of 09:00, 21 October 2021
Wild-type BLP-7-27 ELP
USAGE AND BIOLOGY
We linked elastin like peptide and BLP-7 by a flexible peptide in order to control the molecular size and gather at the lesion. The function of ELP and BLP-7 has been mentioned before.
RESULTS
Figure 1.SDS-PAGE of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP of purification. lane 1/2 is two-tube stream, lane 3 For Wild-type BLP-7-27 ELP, a thicker band appears near the 15kDa marker; lane 4/5/6 is a three-pipe stream that collects Optimized BLP-7-27 ELP, and a thicker band appears near the 15kDa marker.
After culturing, induction and sterilization, the results of nickel column affinity chromatography show that both of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP elution have single peaks at 80 mM with good symmetry, which preliminarily indicates that the target protein is purified. Then we take the BCA method to determine the concentration (Wild-type BLP-7-27 ELP 1.5mg/ml and Optimized BLP-7-27 ELP 1.2mg/ml) and SDS-PAGE to verify the existence of product.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]