Difference between revisions of "Part:BBa K4032003"
Line 13: Line 13:
 
See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for details.
 
See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for details.
  
−
In the case of this part,  red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
+
In the case of this part,  red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.
  
  

Revision as of 08:58, 21 October 2021


amylase-RFP


This part contains the gene of the amylase-RFP fusion protein.

See NCBI: NC_000913.3for information on the amylase gene malS.

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. See NCBI: NC_000913.3for details.

In the case of this part, red fluorescence of RFP is observed with a fluorescence microscope, but isn’t observed under the natural light source.


This part expresses the fusion protein of amylase (from BBa_K523006) and RFP (from BBa_J04450). We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.


The RBS is added to this part, forming BBa_K4032006.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032104.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
    Illegal AgeI site found at 1501
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2692
  • 1000
    COMPATIBLE WITH RFC[1000]