Difference between revisions of "Part:BBa K3827007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | luxCDE sequence is taken from BioBrick BBa_K325909. They are taken together given they form a heterocomplex that functions as a fatty acid reductase (Brodl et al, 2018). | |
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===Source=== | ===Source=== | ||
| − | + | luxCDE sequence is taken from BioBrick BBa_K325909 | |
===References=== | ===References=== | ||
| + | Brodl, E., Winkler, A., & Macheroux, P. (2018). Molecular mechanisms of bacterial bioluminescence. Computational and structural biotechnology journal, 16, 551-564. | ||
Latest revision as of 08:56, 21 October 2021
pBAD - luxCDE -lldR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 4873
Illegal NheI site found at 4896 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3157
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2563
Illegal SapI site found at 961
Illegal SapI.rc site found at 3689
Design Notes
luxCDE sequence is taken from BioBrick BBa_K325909. They are taken together given they form a heterocomplex that functions as a fatty acid reductase (Brodl et al, 2018).
Source
luxCDE sequence is taken from BioBrick BBa_K325909
References
Brodl, E., Winkler, A., & Macheroux, P. (2018). Molecular mechanisms of bacterial bioluminescence. Computational and structural biotechnology journal, 16, 551-564.