Difference between revisions of "Part:BBa K648006"
Menezeslucas (Talk | contribs) |
Isisbnsilva (Talk | contribs) |
||
| Line 7: | Line 7: | ||
This linker was used in a very interesting article, entitled “Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion”. The work addressed the use of the linker in question in the construction of genetic circuits. Being represented as a spacer region in the resulting rtTA-GFP fusion protein, obtained by in-frame insertion of the translated region of yEGFP into the KpnI site of rtTA. It has also been cited in a patent relating to a fc Receptor (fcrn) linking peptides and uses thereof. Where it has been described as a simple 10AA long fusion protein linker comprising glycine and serine residues, it can be used for the formation of cyclic dimeric peptides. <br> | This linker was used in a very interesting article, entitled “Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion”. The work addressed the use of the linker in question in the construction of genetic circuits. Being represented as a spacer region in the resulting rtTA-GFP fusion protein, obtained by in-frame insertion of the translated region of yEGFP into the KpnI site of rtTA. It has also been cited in a patent relating to a fc Receptor (fcrn) linking peptides and uses thereof. Where it has been described as a simple 10AA long fusion protein linker comprising glycine and serine residues, it can be used for the formation of cyclic dimeric peptides. <br> | ||
| − | |||
| − | |||
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 20: | Line 18: | ||
| − | + | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K648006 parameters</partinfo> | <partinfo>BBa_K648006 parameters</partinfo> | ||
| − | < | + | Isoelectric Point: 4,00 <br> |
| + | Molecular Weight: 1071,11Da <br> | ||
| + | |||
| + | |||
| + | ===References=== | ||
| + | |||
| + | #Becskei A, Séraphin B, Serrano L. Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion. EMBO J. 2001;20(10):2528-2535. | ||
Latest revision as of 08:31, 21 October 2021
Long 10AA Fusion Protein Linker with Standard 25 Prefix/Suffix
This is the longest of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the ten amino acids GENLYFQSGG and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.
This linker was used in a very interesting article, entitled “Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion”. The work addressed the use of the linker in question in the construction of genetic circuits. Being represented as a spacer region in the resulting rtTA-GFP fusion protein, obtained by in-frame insertion of the translated region of yEGFP into the KpnI site of rtTA. It has also been cited in a patent relating to a fc Receptor (fcrn) linking peptides and uses thereof. Where it has been described as a simple 10AA long fusion protein linker comprising glycine and serine residues, it can be used for the formation of cyclic dimeric peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
Isoelectric Point: 4,00
Molecular Weight: 1071,11Da
References
- Becskei A, Séraphin B, Serrano L. Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion. EMBO J. 2001;20(10):2528-2535.