Difference between revisions of "Part:BBa K3895016"

Revision as of 08:24, 21 October 2021

Optimized keratinase KerA

Introduction

This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3). The KERA (BBa_K1717171) is a basic part comprised from a synthesized KERA protein coding sequence. The gene can be expressed in cells by looking for clear areas of cell growth on AGAR plates of skimmed milk, and by observing keratin-containing substrates (feathers, hair, chemosynthetic keratin, etc.) growing with cells.

Plasmid Construction

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 326
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 587
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1369
Illegal SapI site found at 957
Illegal SapI site found at 1260

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3895016 short</partinfo>
+==Introduction==
-KERA (<partinfo>K1717171</partinfo>) is a basic part comprised from a synthesized KERA protein coding sequence. This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3).
+This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3). The KERA (<partinfo>K1717171</partinfo>) is a basic part comprised from a synthesized KERA protein coding sequence. The gene can be expressed in cells by looking for clear areas of cell growth on AGAR plates of skimmed milk, and by observing keratin-containing substrates (feathers, hair, chemosynthetic keratin, etc.) growing with cells.
+==Plasmid Construction==
+[[File:T--SZ SHD--kera.png|400px|center]]