Difference between revisions of "Part:BBa K3714004"
Line 13: Line 13:
 
<br/>
 
<br/>
 
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).   
 
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).   
−
<center>[[File:K3714004.png]]</center>
+
<center style="width:90%">[[File:K3714004.png]]</center>
 
<center><b>Figure 1 | Improvement of an existing theophylline aptazyme. a.</b>Cleavage properties of two aptazymes under different theophylline concentration. The sequences were synthesized and transcribed <i>in vitro</i>. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. <b>b. </b>A diagram showing the intensity percentage of the cleaved band of each lane in <b>a</b>. Intensity was measured by ImageJ.</center>
 
<center><b>Figure 1 | Improvement of an existing theophylline aptazyme. a.</b>Cleavage properties of two aptazymes under different theophylline concentration. The sequences were synthesized and transcribed <i>in vitro</i>. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. <b>b. </b>A diagram showing the intensity percentage of the cleaved band of each lane in <b>a</b>. Intensity was measured by ImageJ.</center>
  

Revision as of 08:22, 21 October 2021


Improved theophylline biosensor

An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).

K3714004.png
Figure 1 | Improvement of an existing theophylline aptazyme. a.Cleavage properties of two aptazymes under different theophylline concentration. The sequences were synthesized and transcribed in vitro. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. b. A diagram showing the intensity percentage of the cleaved band of each lane in a. Intensity was measured by ImageJ.