Difference between revisions of "Part:BBa K3722009"

 
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
Biology
 +
TnaA cleaves 6-Br-Trp into 6-Br-indole , pyruvate and ammonia. So we can use this composite part to synthetic 6-Br-indole as the second proceed to product 6,6’-dibromo indigo.
 +
  有图片
 +
 +
Usage
 +
We construct the composite part BBa_K3332052 and transformed it into E. coli BL21 (DE3) to verify its expression.
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 08:08, 21 October 2021


T7_Promoter-RBS-SttH-T7_Terminator

Flavin-dependent Trp halogenases use halide ions (X−) and FADH2 for halogenation，if you use it in cell-free systems, requiring supplementation of the high-cost cofactor FADH2 and additional cofactor regenerating enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1635
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 526
    Illegal AgeI site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]