Difference between revisions of "Part:BBa K4044001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
none
+
Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.
  
  
  
 
===Source===
 
===Source===
 +
BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 and codon optimized for E. coli.
  
 
Source organism: Rhodopseudomonas palustris
 
Source organism: Rhodopseudomonas palustris
  
 
===References===
 
===References===

Revision as of 07:57, 21 October 2021


BphP1 (E.coli optimized)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 246
    Illegal BamHI site found at 1821
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 1153
    Illegal NgoMIV site found at 1650
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.


Source

BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 and codon optimized for E. coli.

Source organism: Rhodopseudomonas palustris

References