Difference between revisions of "Part:BBa K3895016"

 
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<partinfo>BBa_K3895016 short</partinfo>
 
<partinfo>BBa_K3895016 short</partinfo>
  
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KERA (<partinfo>K1717171</partinfo>) is a basic part comprised from a synthesized KERA protein coding sequence. This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3).
  
  

Revision as of 07:53, 21 October 2021


Optimized keratinase KerA

KERA (BBa_K1717171) is a basic part comprised from a synthesized KERA protein coding sequence. This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 326
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1369
    Illegal SapI site found at 957
    Illegal SapI site found at 1260