Difference between revisions of "Part:BBa K3814001"

Revision as of 07:43, 21 October 2021

nahR gene in P. putida + promoter + RBS

The nahR gene activates transcription of the nah and sal operons in response to the inducer salicylate. Traditionally, in the NAH7 plasmid it inhabits, it works in trans of these operons, generating an activator that increases their transcription.

It contains the P-J23100 promoter and the nah3 RBS as well.

Studies have shown that this system works in Escherichia coli with no problem (1,2).

1. Schell MA. Cloning and expression in Escherichia coli of the naphthalene degradation genes from plasmid NAH7. J Bacteriol. 1983 Feb;153(2):822-9. doi: 10.1128/jb.153.2.822-829.1983. PMID: 6296054; PMCID: PMC221701.

2. Schell MA, Wender PE. Identification of the nahR gene product and nucleotide sequences required for its activation of the sal operon. J Bacteriol. 1986 Apr;166(1):9-14. doi: 10.1128/jb.166.1.9-14.1986. PMID: 3007442; PMCID: PMC214548.

Secondary Structure

File:Mfold-K3814001-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

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-* The nahR gene activates transcription of the nah and sal operons in response to the inducer salicylate. Traditionally in the NAH7 plasmid it inhabits, it works in trans of these operons, generating an activator that increases their transcription.
+* The nahR gene activates transcription of the nah and sal operons in response to the inducer salicylate. Traditionally, in the NAH7 plasmid it inhabits, it works in trans of these operons, generating an activator that increases their transcription.
 It contains the P-J23100 promoter and the nah3 RBS as well.