Difference between revisions of "Part:BBa K3765008"

Latest revision as of 07:08, 21 October 2021

OpdA

Phosphotriesterase enzyme from Agrobacterium tumefaciens, which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry BBa_K215090, this part also encodes the 17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.

Usage and Biology

This part was of major importance in our project. The OpdA enzyme can degrade a wide range of organophosphates and since our project is aimed at building a filter to fight organophosphate pollution, we constructed an OpdA-SpyTag002 fusion protein to functionalize a microcrystalline cellulose sheet-based filter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3765008 short</partinfo>
-Phosphotriesterase enzyme.
+Phosphotriesterase enzyme from ''Agrobacterium tumefaciens'', which degrades organophosphates which are triesters of phosphoric acid. Unlike the previous OpdA part on the Registry [https://parts.igem.org/Part:BBa_K215090 BBa_K215090], this part also encodes the  17 amino acid long tail at the C-terminus of the enzyme which may be useful for construction of C-terminal fusion proteins using this part.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part was of major importance in our project. The OpdA enzyme can degrade a wide range of organophosphates and since our project is aimed at building a filter to fight organophosphate pollution, we constructed an OpdA-SpyTag002 fusion protein to functionalize a microcrystalline cellulose sheet-based filter.
+----
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3765008 SequenceAndFeatures</partinfo>