Difference between revisions of "Part:BBa K3874003"

Latest revision as of 06:01, 21 October 2021

To add Linker between single repeat

This part is to code a sericin like protein. The sequence of this protein has many repeat and this part is the 8 repeat unit. We add BBa_K243004(GGSG), a linker between each Sericin repeat. The protein will be folded well with the linker.

Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.

Figure 2. Western Blot Data prove IPTG 0.5mM in 18℃ and cultivating for 20h is the most proper condition and we use this condition in latter experiment..

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3874003 short</partinfo>
-We add BBa_K243004(GGSG), a linker between our Sericin repeat. The protein will be folded well with the linker.
+This part is to code a sericin like protein. The sequence of this protein has many repeat and this part is the 8 repeat unit.
+We add BBa_K243004(GGSG), a linker between each Sericin repeat. The protein will be folded well with the linker.
+[[File:T--BJ101ID--3.png|400px|thumb|left|Figure 1. The Schematic cartoon of the DNA construct of BBa_K3874001.]]
+[[File:T--BJ101ID--4.png|400px|thumb|left|Figure 2. Western Blot Data prove IPTG 0.5mM in 18℃ and cultivating for 20h is the most proper condition and we use this condition in latter experiment..]]
 <!-- Add more about the biology of this part here