Difference between revisions of "Part:BBa K4032003"
Line 3: Line 3:
 
<partinfo>BBa_K4032003 short</partinfo>
 
<partinfo>BBa_K4032003 short</partinfo>
  
−
NC_000913.3 https://www.ncbi.nlm.nih.gov/gene/948088
 
  
 
This part contains the gene of the amylase-RFP fusion protein.
 
This part contains the gene of the amylase-RFP fusion protein.
  
 +
See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for information on the amylase gene ''malS''.
  
 
===Usage and Biology===
 
===Usage and Biology===
  
−
This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
+
This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units.
 +
See [https://www.ncbi.nlm.nih.gov/gene/948088 NCBI: NC_000913.3]for details.
  
 
In the case of this part,  red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
 
In the case of this part,  red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
  
  
−
This part expresses the fuse protein of amylase (from <partinfo>BBa_K523006</partinfo>) and RFP (from <partinfo>BBa_J04450</partinfo>). We removed the stop codon of amylase and the start codon of RFP and connected the ends.
+
This part expresses the fusion protein of amylase (from <partinfo>BBa_K523006</partinfo>) and RFP (from <partinfo>BBa_J04450</partinfo>). We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.
  
  
−
The RBS is added to this part forming <partinfo>BBa_K4032006</partinfo>.
+
The RBS is added to this part, forming <partinfo>BBa_K4032006</partinfo>.
  
−
The lac promoter, the RBS and the double terminator are added to this part forming <partinfo>BBa_K4032104</partinfo>.
+
The lac promoter, the RBS and the double terminator are added to this part, forming <partinfo>BBa_K4032104</partinfo>.
  
  

Revision as of 05:56, 21 October 2021


amylase-RFP


This part contains the gene of the amylase-RFP fusion protein.

See NCBI: NC_000913.3for information on the amylase gene malS.

Usage and Biology

This enzyme hydrolyzes of (1-4)-α-D-glycosidic linkages in polysaccharides containing three or more (1-4)-α-linked D-glucose units. See NCBI: NC_000913.3for details.

In the case of this part, red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.


This part expresses the fusion protein of amylase (from BBa_K523006) and RFP (from BBa_J04450). We removed the stop codon of the amylase gene and the start codon of the RFP gene and connected the two ends.


The RBS is added to this part, forming BBa_K4032006.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032104.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
    Illegal AgeI site found at 1501
    Illegal AgeI site found at 2580
    Illegal AgeI site found at 2692
  • 1000
    COMPATIBLE WITH RFC[1000]