Difference between revisions of "Part:BBa K3722004"
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===Biology=== | ===Biology=== | ||
| − | + | After linked the Fre to SttH, the total expression of SttH decreased, but all the chimeric enzymes showed highly increased ratios of their soluble forms in E. coli. The molar concentrations of soluble Fre-Linker–SttH was 1.64 μM, which was more than three times higher than that of SttH by itself[1]. | |
===Usage=== | ===Usage=== | ||
Revision as of 05:18, 21 October 2021
A linker sequence in fusion protein Fre-Linker-SttH
A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.
Biology
After linked the Fre to SttH, the total expression of SttH decreased, but all the chimeric enzymes showed highly increased ratios of their soluble forms in E. coli. The molar concentrations of soluble Fre-Linker–SttH was 1.64 μM, which was more than three times higher than that of SttH by itself[1].
Usage
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]