Difference between revisions of "Part:BBa K176007"

Latest revision as of 09:06, 16 June 2009

pSB1A3 with the suffix of J61002 (mRFP)

It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbone J61002.

SpeI-RBS-mRFP-Terminator-NotI-PstI-pSB1A3-prefix

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3003
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal NotI site found at 878
Illegal NotI site found at 3009
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3003
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3003
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 885
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3003
Plasmid lacks a suffix.
Illegal XbaI site found at 3018
Illegal SpeI site found at 2
Illegal PstI site found at 885
Illegal AgeI site found at 581
Illegal AgeI site found at 693
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2042

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K176007 short</partinfo>
-It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002.
+It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbone J61002.
-SpeI-RBS-mRFP-Terminator-PstI-pSB1A3-prefix
+SpeI-RBS-mRFP-Terminator-NotI-PstI-pSB1A3-prefix
 <!-- Add more about the biology of this part here