Difference between revisions of "Part:BBa K3796005"
 
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<partinfo>BBa_K3796005 short</partinfo>
 
<partinfo>BBa_K3796005 short</partinfo>
  
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The RBS sequence, or ribosome binding site, is located several sequences upstream of the gene expression frame and is an important sequence for pre-translational recognition and binding of the ribosome. When the DNA fragment downstream of the promoter and upstream of the terminator is transcribed out of the mRNA, the ribosome recognizes and binds the RBS sequence on the mRNA and then scans down until it recognizes the start codon and begins translation.
 
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===Usage and Biology===
 
 
This is a weak strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can decreasing gene expression by reducing translation in Corynebacterium glutamicum.
 
This is a weak strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can decreasing gene expression by reducing translation in Corynebacterium glutamicum.
 
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Latest revision as of 03:44, 21 October 2021


Corynebacterium glutamicum weak RBS

This is a weak strength RBS, which is optimized from the original ones in Corynebacterium glutamicum and can decreasing gene expression by reducing translation in Corynebacterium glutamicum.
By applying the weak strength RBS, reducing gene expression be made through regulation on the translation level. It has been verified that this RBS can realize reduction in Corynebacterium glutamicum. However, application in other species is not clear yet. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]