Difference between revisions of "Help:Plasmid Backbone Availability 2009"
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| − | |'''pSB1A3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205] | + | |'''pSB1A3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1A3 Kit Plate 1, well 1K]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1A3 Kit Plate 1, well 3K]</span> || Kit Plate 2, well 10G [https://parts.igem.org/partsdb/get_part.cgi?id=4586&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I732205 BBa_I732205] |
|- | |- | ||
| − | |'''pSB1AC3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001] | + | |'''pSB1AC3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AC3 Kit Plate 1, well 11A]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AC3 Kit Plate 1, well 11C]</span> || Kit Plate 2, well 22M [https://parts.igem.org/partsdb/get_part.cgi?id=5485&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_M1001 BBa_M1001] |
|- | |- | ||
|'''pSB1AK3''' || NO || NO || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053] | |'''pSB1AK3''' || NO || NO || Kit Plate 3, well 20A [https://parts.igem.org/partsdb/get_part.cgi?id=4942&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K143053 BBa_K143053] | ||
|- | |- | ||
| − | |'''pSB1AT3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001] | + | |'''pSB1AT3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AT3 Kit Plate 1, well 15A]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AT3 Kit Plate 1, well 15C]</span> || Kit Plate 3, well 1B [https://parts.igem.org/partsdb/get_part.cgi?id=5483&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K115001 BBa_K115001] |
|- | |- | ||
|'''pSB1A10''' || NO || NO || NO || | |'''pSB1A10''' || NO || NO || NO || | ||
|- | |- | ||
| − | |'''pSB4A5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || | + | |'''pSB4A5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4A5 Kit Plate 1, well 3M]</span> || NO || |
|- | |- | ||
|'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001] | |'''pSB6A1''' || NO || NO || Kit Plate 2, well 14G [https://parts.igem.org/partsdb/get_part.cgi?id=10245&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I739001 BBa_I739001] | ||
|- | |- | ||
| − | |'''pSB1C3''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || | + | |'''pSB1C3''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1C3 Kit Plate 1, well 5A]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1C3 Kit Plate 1, well 5E]</span> || NO || |
|- | |- | ||
| − | |'''pSB3C5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || | + | |'''pSB3C5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3C5 Kit Plate 1, well 5G]</span> || NO || |
|- | |- | ||
| − | |'''pSB4C5''' || NO || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010] | + | |'''pSB4C5''' || NO || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4C5 Kit Plate 1, well 5I]</span> || Kit Plate 3, well 5F [https://parts.igem.org/partsdb/get_part.cgi?id=8151&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K098010 BBa_K098010] |
|- | |- | ||
| − | |'''pSB1K3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO || | + | |'''pSB1K3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1K3 Kit Plate 1, well 7A]</span> || NO || NO || |
|- | |- | ||
|'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033] | |'''pSB2K3''' || NO || NO || Kit Plate 2, well 11B [https://parts.igem.org/partsdb/get_part.cgi?id=4584&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_E0033 BBa_E0033] | ||
| Line 44: | Line 44: | ||
|'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891] | |'''pSB3K3''' || NO || NO || Kit Plate 2, well 15L [https://parts.igem.org/partsdb/get_part.cgi?id=4739&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I714891 BBa_I714891] | ||
|- | |- | ||
| − | |'''pSB4K5''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003] | + | |'''pSB4K5''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB4K5 Kit Plate 1, well 7G]</span> || NO || Kit Plate 2, well 17H [https://parts.igem.org/partsdb/get_part.cgi?id=8325&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_T9003 BBa_T9003] |
|- | |- | ||
| − | |'''pSB1AK8''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006] | + | |'''pSB1AK8''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1AK8 Kit Plate 1, well 13C]</span> || NO || Kit Plate 2, well 13A [https://parts.igem.org/partsdb/get_part.cgi?id=7524&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I712006 BBa_I712006] |
|- | |- | ||
| − | |'''pSB1T3''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO || | + | |'''pSB1T3''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB1T3 Kit Plate 1, well 9A]</span> || NO || NO || |
|- | |- | ||
| − | |'''pSB3T5''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045] | + | |'''pSB3T5''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3T5 Kit Plate 1, well 9C]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=pSB3T5 Kit Plate 1, well 9G]</span> || Kit Plate 2, well 19A [https://parts.igem.org/partsdb/get_part.cgi?id=8615&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I715045 BBa_I715045] |
|- | |- | ||
|'''pSB4T5''' || NO || NO || NO || | |'''pSB4T5''' || NO || NO || NO || | ||
|- | |- | ||
| − | |'''BBa_I51020''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || | + | |'''BBa_I51020''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I51020 Kit Plate 1, well 1A]</span> || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I51020 Kit Plate 1, well 3A]</span> || NO || |
|- | |- | ||
| − | |'''BBa_I716101''' || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || NO || NO || | + | |'''BBa_I716101''' || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_I716101 Kit Plate 1, well 1C]</span> || NO || NO || |
|- | |- | ||
| − | |'''BBa_J63009''' || YES <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || YES [?] <br> <span style="font-size:75%;">[ Kit Plate 1, well ]</span> || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018] | + | |'''BBa_J63009''' || YES <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J63009 Kit Plate 1, well 1E]</span> || YES [?] <br> <span style="font-size:75%;">[https://parts.igem.org/partsdb/get_part.cgi?part=BBa_J63009 Kit Plate 1, well 3E]</span> || Kit Plate 3, well 2M [https://parts.igem.org/partsdb/get_part.cgi?id=7521&show=1 [more]]|| [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165018 BBa_K165018] |
|- | |- | ||
|'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001] | |'''BBa_J63010''' || NO || NO || Kit Plate 3, well 2K [https://parts.igem.org/partsdb/get_part.cgi?id=7585&show=1 [more]] || [https://parts.igem.org/partsdb/get_part.cgi?part=BBa_K165001 BBa_K165001] | ||
Revision as of 14:51, 15 June 2009
**EDITING IN PROGRESS**
The following is a list of plasmid backbones that we have included as part of the 2009 distribution kit, most of which have P1010 and J04450. However, if you are having trouble with these, we have also included alternate sources for the plasmids.
- YES - The plasmid is available and correct
- YES[?] - The plasmid is available and correct, with the exception of one inconsistency in the QC results which should not be an issue
- NO - The plasmid is either unavailable or we have found that it carries an error
| Plasmid | J04450 | P1010 | Alt. Location | Insert (click for QC) |
|---|---|---|---|---|
| pSB1A3 | YES Kit Plate 1, well 1K |
YES Kit Plate 1, well 3K |
Kit Plate 2, well 10G [more] | BBa_I732205 |
| pSB1AC3 | YES [?] Kit Plate 1, well 11A |
YES [?] Kit Plate 1, well 11C |
Kit Plate 2, well 22M [more] | BBa_M1001 |
| pSB1AK3 | NO | NO | Kit Plate 3, well 20A [more] | BBa_K143053 |
| pSB1AT3 | YES Kit Plate 1, well 15A |
YES [?] Kit Plate 1, well 15C |
Kit Plate 3, well 1B [more] | BBa_K115001 |
| pSB1A10 | NO | NO | NO | |
| pSB4A5 | NO | YES Kit Plate 1, well 3M |
NO | |
| pSB6A1 | NO | NO | Kit Plate 2, well 14G [more] | BBa_I739001 |
| pSB1C3 | YES Kit Plate 1, well 5A |
YES Kit Plate 1, well 5E |
NO | |
| pSB3C5 | NO | YES Kit Plate 1, well 5G |
NO | |
| pSB4C5 | NO | YES Kit Plate 1, well 5I |
Kit Plate 3, well 5F [more] | BBa_K098010 |
| pSB1K3 | YES [?] Kit Plate 1, well 7A |
NO | NO | |
| pSB2K3 | NO | NO | Kit Plate 2, well 11B [more] | BBa_E0033 |
| pSB3K3 | NO | NO | Kit Plate 2, well 15L [more] | BBa_I714891 |
| pSB4K5 | YES [?] Kit Plate 1, well 7G |
NO | Kit Plate 2, well 17H [more] | BBa_T9003 |
| pSB1AK8 | YES [?] Kit Plate 1, well 13C |
NO | Kit Plate 2, well 13A [more] | BBa_I712006 |
| pSB1T3 | YES [?] Kit Plate 1, well 9A |
NO | NO | |
| pSB3T5 | YES [?] Kit Plate 1, well 9C |
YES [?] Kit Plate 1, well 9G |
Kit Plate 2, well 19A [more] | BBa_I715045 |
| pSB4T5 | NO | NO | NO | |
| BBa_I51020 | YES Kit Plate 1, well 1A |
YES Kit Plate 1, well 3A |
NO | |
| BBa_I716101 | YES [?] Kit Plate 1, well 1C |
NO | NO | |
| BBa_J63009 | YES Kit Plate 1, well 1E |
YES [?] Kit Plate 1, well 3E |
Kit Plate 3, well 2M [more] | BBa_K165018 |
| BBa_J63010 | NO | NO | Kit Plate 3, well 2K [more] | BBa_K165001 |
- If you decide to use an alternate location for the plasmid, keep in mind that after transforming and miniprepping you should use PCR to make the construction plasmid. This step will produce PCR product containing the plasmid backbone with no insert and no uncut, circular plasmid, and can then be cut with the restriction enzymes for use.
We will continue to update this page with more information and details, including more comprehensive quality control information and alternate locations.