Difference between revisions of "Part:BBa K4074045:Design"

 
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===Source===
 
===Source===
  
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-
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pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab
  
 
===References===
 
===References===

Latest revision as of 03:22, 21 October 2021


pSB_BKD_GIBSON


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3503
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 2317
    Illegal PstI site found at 2305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 2344
    Illegal SpeI site found at 3504
    Illegal PstI site found at 2305
    Illegal PstI site found at 3518
    Illegal NotI site found at 1193
    Illegal NotI site found at 3492
    Illegal NotI site found at 3511
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 2344
    Illegal BamHI site found at 2323
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3504
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 2317
    Illegal PstI site found at 2305
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2344
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 2317
    Illegal SpeI site found at 3504
    Illegal PstI site found at 2305
    Illegal PstI site found at 3518
    Illegal NgoMIV site found at 3463
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References