Difference between revisions of "Part:BBa K4074043"

 
 
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<partinfo>BBa_K4074043 short</partinfo>
 
<partinfo>BBa_K4074043 short</partinfo>
  
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<b> Contents: </b> AmyE homology arms (Gibson) <br>
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<b> What is it?: </b> A basic part - plasmid backbone <br>
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<b> What does it do?: </b> It codes for amyE homology arms, which allow for integration into the amyE locus of the B subtilis chromosome. <br>
 
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<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 03:13, 21 October 2021


pSB_AMY_GIBSON

Contents: AmyE homology arms (Gibson)
What is it?: A basic part - plasmid backbone
What does it do?: It codes for amyE homology arms, which allow for integration into the amyE locus of the B subtilis chromosome.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3886
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 2450
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal SpeI site found at 3887
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
    Illegal PstI site found at 3901
    Illegal NotI site found at 1193
    Illegal NotI site found at 3875
    Illegal NotI site found at 3894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal BamHI site found at 2456
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3887
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 2450
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1772
    Illegal EcoRI site found at 2477
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 2450
    Illegal SpeI site found at 3887
    Illegal PstI site found at 1960
    Illegal PstI site found at 1986
    Illegal PstI site found at 2438
    Illegal PstI site found at 3901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.