Difference between revisions of "Part:BBa K3783001:Experience"
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===Applications of BBa_K3783001=== | ===Applications of BBa_K3783001=== | ||
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=OhioState Application= | =OhioState Application= | ||
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<img src="https://2021.igem.org/wiki/images/1/1b/T--OhioState--pR-pFraB.jpg" class="generalimage" alt="pR-pFraB Graph"> | <img src="https://2021.igem.org/wiki/images/1/1b/T--OhioState--pR-pFraB.jpg" class="generalimage" alt="pR-pFraB Graph"> | ||
| − | <figcaption style="text-align:center">Figure | + | <figcaption style="text-align:center">Figure 1. pR-pFraB Luciferase Reporter</figcaption> |
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| − | + | <p> The promoter was cloned into a luciferase reporter plasmid and then transformed into a fraR expressing strain of <i>E.coli</i>. The repressor protein has been expressed under the lacZ promoter thus addition of IPTG results in increased amounts of repressor. The luminescence curve of the fusion promoter follows mostly as expected. There is a significant, almost threefold, repression of production of target proteins in the fraR+ strains. However, due to the presence of the pR, the proteins are produced at a massive scale, in the millions of light units. | |
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Applications of BBa_K3783001
OhioState Application
The promoter was cloned into a luciferase reporter plasmid and then transformed into a fraR expressing strain of E.coli. The repressor protein has been expressed under the lacZ promoter thus addition of IPTG results in increased amounts of repressor. The luminescence curve of the fusion promoter follows mostly as expected. There is a significant, almost threefold, repression of production of target proteins in the fraR+ strains. However, due to the presence of the pR, the proteins are produced at a massive scale, in the millions of light units.
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