Difference between revisions of "Part:BBa K3714004"
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<partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3714004 SequenceAndFeatures</partinfo> | ||
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| + | By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1). | ||
<center>[[File:K3714004.png]]</center> | <center>[[File:K3714004.png]]</center> | ||
| − | <center><b>Figure | + | <center><b>Figure 1 | Improvement of an existing theophylline aptazyme. a.</b>Cleavage properties of two aptazymes under different theophylline concentration. The sequences was synthesized and transcribed <i>in vitro</i>. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. <b>b. </b>A diagram showing the intensity percentage of the cleaved band of each lane in <b>a</b>. Intensity was measured by ImageJ.</center> |
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Revision as of 02:45, 21 October 2021
Improved theophylline biosensor
An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).
