Difference between revisions of "Part:BBa K3783000:Experience"
(→OhioState Application) |
(→OhioState Application) |
||
| (2 intermediate revisions by one other user not shown) | |||
| Line 17: | Line 17: | ||
</style> | </style> | ||
| − | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli/i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of | + | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression. |
<body> | <body> | ||
Latest revision as of 02:39, 21 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3783000
OhioState Application
The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.
User Reviews
UNIQ7e991f10107cf0ed-partinfo-00000001-QINU UNIQ7e991f10107cf0ed-partinfo-00000002-QINU