Difference between revisions of "Part:BBa K3783000:Experience"
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| + | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression. | ||
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<img src="https://2021.igem.org/wiki/images/a/a6/T--OhioState--pFraB.jpg" class="generalimage" alt="pFraB Graph"> | <img src="https://2021.igem.org/wiki/images/a/a6/T--OhioState--pFraB.jpg" class="generalimage" alt="pFraB Graph"> | ||
| − | <figcaption style="text-align:center">Figure | + | <figcaption style="text-align:center">Figure 1. pFraB Luciferase Reporter</figcaption> |
</figure> | </figure> | ||
</body> | </body> | ||
Latest revision as of 02:39, 21 October 2021
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Applications of BBa_K3783000
OhioState Application
The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.
User Reviews
UNIQb5c08640749fcbfa-partinfo-00000001-QINU UNIQb5c08640749fcbfa-partinfo-00000002-QINU