Difference between revisions of "Part:BBa K3783000:Experience"
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=OhioState Application=
 
=OhioState Application=
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<p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.
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<img src="https://2021.igem.org/wiki/images/a/a6/T--OhioState--pFraB.jpg" class="generalimage" alt="pFraB Graph">
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<figcaption style="text-align:center">Figure 1. pFraB Luciferase Reporter</figcaption>
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===User Reviews===
 
===User Reviews===

Latest revision as of 02:39, 21 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3783000

OhioState Application

The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.

pFraB Graph
Figure 1. pFraB Luciferase Reporter

User Reviews

UNIQd6ef5d02bc5bdca4-partinfo-00000001-QINU UNIQd6ef5d02bc5bdca4-partinfo-00000002-QINU