Difference between revisions of "Part:BBa K3783000:Experience"
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| − | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli | + | <p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression. |
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Revision as of 02:36, 21 October 2021
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Applications of BBa_K3783000
OhioState Application
The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression.
User Reviews
UNIQ60d12bef20360f28-partinfo-00000001-QINU UNIQ60d12bef20360f28-partinfo-00000002-QINU