Difference between revisions of "Part:BBa K3783000:Experience"

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<p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli/<i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression.  
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<p> The promoter was cloned into a luciferase reporter and then transformed into a strain of <i>E.coli</i> expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression.  
  
 
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Revision as of 02:36, 21 October 2021


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Applications of BBa_K3783000

OhioState Application

The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling expression of expressed proteins. The fraR repressor was expressed via the lacZ promoter. This is why addition of IPTG results in increased repression.

pFraB Graph
Figure 1. pFraB Luciferase Reporter

User Reviews

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