Difference between revisions of "Part:BBa K3714004"
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<center>[[File:K3714004.png]]</center>
 
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<center><b>Figure.1 | Verification of biosensor gard-337. </b> An in vitro transcription system was employed to test if this biosensor can response gardiquimod signal. The product was run on a denatured PAGE. </center>
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<center><b>Figure.1 | Improvement of an existing theophylline aptazyme. a.</b>Cleavage properties of two aptazymes under different theophylline concentration. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. <b>b. </b>A diagram showing the intensity percentage of the cleaved band of each lane in <b>a</b>. Intensity was measured by ImageJ.</center>
  
 
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Revision as of 02:32, 21 October 2021


Improved theophylline biosensor

An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714004.png
Figure.1 | Improvement of an existing theophylline aptazyme. a.Cleavage properties of two aptazymes under different theophylline concentration. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. b. A diagram showing the intensity percentage of the cleaved band of each lane in a. Intensity was measured by ImageJ.