Difference between revisions of "Part:BBa K3790004"
TiTong Fudan (Talk | contribs) (→Introduction) |
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− | Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase. | + | Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.albA1, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments. |
− | The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments | + | |
Revision as of 01:14, 21 October 2021
albA1
Introduction
Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.albA1, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments.
Usage and Biology
Experimental Results
Reference
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]