Difference between revisions of "Part:BBa K3806016:Design"

(Replaced content with " __NOTOC__ <partinfo>BBa_K3806016 short</partinfo> <partinfo>BBa_K3806016 SequenceAndFeatures</partinfo> ===Design Notes=== prov ===Source=== prov ===References===")
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
prov
+
This biobrick represents the DNA template for the expression of the <i>lacZ</i> reporter gene in a T7 based cell-free expression system. Expression is regulated by a theophylline binding aptazyme. The dsRNA sequence was PCR amplified from BBa_K3806014, with a primer that changes the C and T (U in the aptazyme) nucleotides of the antisense strand into G and A, respectively, resulting in a partial mismatch to the RBS (Fig. 1).
  
 +
Fwd primer: 5’ AATTTAATACGACTCACTATAGGGAAACAAACAAA<b>GA</b>CCTTGCTGTCACCGGAATG 3’
 +
Rv primer: 5’ CACACAGGAAACAGCTATGACCATG 3’
  
  
  
−
===Source===
 
−
 
−
prov
 
  
 
===References===
 
===References===

Revision as of 00:33, 21 October 2021


Theophylline-binding aptazyme regulating lacZ expression (semi-cRBS). With T7 promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2321
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick represents the DNA template for the expression of the lacZ reporter gene in a T7 based cell-free expression system. Expression is regulated by a theophylline binding aptazyme. The dsRNA sequence was PCR amplified from BBa_K3806014, with a primer that changes the C and T (U in the aptazyme) nucleotides of the antisense strand into G and A, respectively, resulting in a partial mismatch to the RBS (Fig. 1).

Fwd primer: 5’ AATTTAATACGACTCACTATAGGGAAACAAACAAAGACCTTGCTGTCACCGGAATG 3’ Rv primer: 5’ CACACAGGAAACAGCTATGACCATG 3’



References