Difference between revisions of "Part:BBa K3714000"
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<partinfo>BBa_K3714000 short</partinfo> | <partinfo>BBa_K3714000 short</partinfo> | ||
− | The template of a newly found self-cleaving aptazyme[1], which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod. | + | The template of a newly found self-cleaving aptazyme[1], which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod. The feature "Aptazyme" is necessary, and two A-rich oligonucleotides are added at both terminal to avoid unwanted secondary structure. |
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===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 23:12, 20 October 2021
Gardiquimod biosensor 337
The template of a newly found self-cleaving aptazyme[1], which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod. The feature "Aptazyme" is necessary, and two A-rich oligonucleotides are added at both terminal to avoid unwanted secondary structure.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] Townshend, B., Xiang, J. S., Manzanarez, G., Hayden, E. J. & Smolke, C. D. A multiplexed, automated evolution pipeline enables scalable discovery and characterization of biosensors. Nature Communications 12, 1437, doi:10.1038/s41467-021-21716-0 (2021).