Difference between revisions of "Part:BBa K4046050"
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Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349. | Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349. | ||
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Revision as of 22:33, 20 October 2021
Kozak Sequence (Human RBS)
This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.
Design Notes
The start codon ATG is not included in this version of the Kozak sequence.
Source
The Kozak sequence was determined through analysis of various eukaryotic mRNAs.
References
Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349.