Difference between revisions of "Part:BBa K4046050"

Revision as of 22:32, 20 October 2021

Kozak Sequence (Human RBS)

This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.

Design Notes

The start codon ATG is not included in this version of the Kozak sequence.

Source

The Kozak sequence was determined through analysis of various eukaryotic mRNAs.

References

Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is the Kozak sequence used by the 2021 Duke iGEM team for constructing our plasmids. The Kozak sequence is meant to facilitate the proper initiation of translation in eukaryotic mRNA transcripts. The Kozak sequence is inserted immediately upstream of our reporter protein within each construct.
-<!-- Add more about the biology of this part here
+===Design Notes===
-===Usage and Biology===
+The start codon ATG is not included in this version of the Kozak sequence.
+===Source===
+The Kozak sequence was determined through analysis of various eukaryotic mRNAs.
+===References===
+Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125. PMID: 3313277; PMCID: PMC306349.
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