Difference between revisions of "Part:BBa K4080007"
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<partinfo>BBa_K4080007 short</partinfo> | <partinfo>BBa_K4080007 short</partinfo> | ||
− | (edit) | + | (edit) This composite is using a constitutive family promoter and artificial terminator to enable expression under non T7 vectors. The feasibility of the experiment will be tested by overexpressing Chromoproteins, and by utilizing the biterminal display scaffold eCPX will they be localized on the outer membrane of the E.coli host. This cassette system is one of two that will be working to generate a CHOP system. Characterization of two expression systems (between T7 and non T7) is measured to select the best blueprint for the CHOP system. |
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Revision as of 22:30, 20 October 2021
amilCP-eCPX fusion with universal expression
(edit) This composite is using a constitutive family promoter and artificial terminator to enable expression under non T7 vectors. The feasibility of the experiment will be tested by overexpressing Chromoproteins, and by utilizing the biterminal display scaffold eCPX will they be localized on the outer membrane of the E.coli host. This cassette system is one of two that will be working to generate a CHOP system. Characterization of two expression systems (between T7 and non T7) is measured to select the best blueprint for the CHOP system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]