Difference between revisions of "Part:BBa K4047021:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This sequence has limited compatibility, and we were not able to explore silent mutations that could improve compatibility within the competition timeframe. We strongly encourage future teams to retain optimization for photosynthetic chassis organisms while improving compatibility. | |
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===Source=== | ===Source=== | ||
| − | + | This terminator was part of an established kanamycin resistance cassette in the Miami University Wang microbiology lab for use in Synechococcus elongatus PCC 7942. | |
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===References=== | ===References=== | ||
Latest revision as of 21:00, 20 October 2021
Kanamycin Resistance Terminator for Synechococcus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 9
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence has limited compatibility, and we were not able to explore silent mutations that could improve compatibility within the competition timeframe. We strongly encourage future teams to retain optimization for photosynthetic chassis organisms while improving compatibility.
Source
This terminator was part of an established kanamycin resistance cassette in the Miami University Wang microbiology lab for use in Synechococcus elongatus PCC 7942.