Difference between revisions of "Part:BBa K4047021"

Latest revision as of 20:58, 20 October 2021

Kanamycin Resistance Terminator for Synechococcus

This terminator allows the transcription and expression of kanamycin resistance (KanR) in Synechococcus elongatus PCC 7942, optimized for this photosynthetic chassis as opposed to other bacteria. Specifically, this part was created in conjunction with the kanamycin resistance coding sequence (BBa_K4047019) and the kanamycin promoter sequence (BBa_K4047020) as part of the plasmid BBa_K4047039.

This part was functional in wet lab experiments, allowing transcription of functional kanamycin resistance and growth of transformed colonies on kanamycin-containing BG11 plates.

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

@@ Line 1: / Line 1: @@
 __NOTOC__
-{|width='80%'
-|-
-|width=35% valign='top'|
-[[Image:Terminator.png]]
-<partinfo>BBa_K4047021 parameters</partinfo>
-| width=50% valign='top' style='border: 1px solid black'|
 <partinfo>BBa_K4047021 short</partinfo>
-* Sequence marks the end of KanR transcription
-|}
+This terminator allows the transcription and expression of kanamycin resistance (KanR) in Synechococcus elongatus PCC 7942, optimized for this photosynthetic chassis as opposed to other bacteria. Specifically, this part was created in conjunction with the kanamycin resistance coding sequence (<partinfo>BBa_K4047019</partinfo>) and the kanamycin promoter sequence (<partinfo>BBa_K4047020</partinfo>) as part of the plasmid <partinfo>BBa_K4047039</partinfo>.
+This part was functional in wet lab experiments, allowing transcription of functional kanamycin resistance and growth of transformed colonies on kanamycin-containing BG11 plates.
-'''Secondary Structure'''
-[[Image:Mfold-K4047021-1.png]]
 <hr>
 '''Measurement'''
 * [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]