Difference between revisions of "Part:BBa K3783000"
 
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The fraB promoter is a regulatory sequence found in Salmonella where it serves an important role in the regulation of the metabolism of fructose-asparagine. In the absence of fructose-asparagine, another regulatory gene, fraR, is activated, and binds to pFraB which represses downstream expression. In the presence of fructose-asparagine, or with the absence of a fraR gene, pFraB is active.
 
The fraB promoter is a regulatory sequence found in Salmonella where it serves an important role in the regulation of the metabolism of fructose-asparagine. In the absence of fructose-asparagine, another regulatory gene, fraR, is activated, and binds to pFraB which represses downstream expression. In the presence of fructose-asparagine, or with the absence of a fraR gene, pFraB is active.
  
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<img src="https://2021.igem.org/wiki/images/a/a6/T--OhioState--pFraB.jpg" class="generalimage" alt="pFraB Graph">
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<figcaption style="text-align:center">Figure 2. pFraB Luciferase Reporter</figcaption>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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Strong Promoter
  
 
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<span class='h3bb'>Sequence and Features: </span>
 
<span class='h3bb'>Sequence and Features: </span>
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Native RBS is included from <i i> Salmonella
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Native RBS is included from <i>Salmonella
 
<partinfo>BBa_K3783000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3783000 SequenceAndFeatures</partinfo>
  

Latest revision as of 20:24, 20 October 2021


pFraB Promoter

The fraB promoter is a regulatory sequence found in Salmonella where it serves an important role in the regulation of the metabolism of fructose-asparagine. In the absence of fructose-asparagine, another regulatory gene, fraR, is activated, and binds to pFraB which represses downstream expression. In the presence of fructose-asparagine, or with the absence of a fraR gene, pFraB is active.


Usage and Biology

Strong Promoter

Sequence and Features: Native RBS is included from Salmonella


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]