Difference between revisions of "Part:BBa K4047019"

 
 
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<partinfo>BBa_K4047019 short</partinfo>
 
<partinfo>BBa_K4047019 short</partinfo>
  
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Sequence used within our dSBPase plasmid encoding kanamycin resistance (KanR) to serve as a selective marker.  
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Confers resistance to the antibiotic kanamycin. This sequence was used within the plasmid <partinfo>BBa_K4047039</partinfo>. This plasmid, which deletes the target gene glpX by insertion of kanamycin, simultaneously using this resistance-coding gene (KanR) as a selective marker for successful transformation.
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Colonies were grown on BG11 media containing kanamycin. PCR was used to verify the change in genomic content, with an increasing band width indicating successful integration of the kanamycin resistance gene into the target site. See the Experience tab for more details on the successful operation of this part in the MiamiU_OH project.
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This part is only the coding region for kanamycin resistance, with the promoter <partinfo>BBa_K4047020</partinfo> and terminator <partinfo>BBa_K4047021</partinfo> being vital accessory components to its functional operation.
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This part is an improvement to the documented part  <partinfo>K1424003</partinfo>, which was optimized for Synechocystis sp. PCC 6803. This has been optimized here for use in the organism Synechococcus elongatus PCC 7942, and as such has a different DNA sequence.  
  
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===Usage and Biology===
 
  
 
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Latest revision as of 20:05, 20 October 2021


Kanamycin Resistance for Synechococcus

Confers resistance to the antibiotic kanamycin. This sequence was used within the plasmid BBa_K4047039. This plasmid, which deletes the target gene glpX by insertion of kanamycin, simultaneously using this resistance-coding gene (KanR) as a selective marker for successful transformation.

Colonies were grown on BG11 media containing kanamycin. PCR was used to verify the change in genomic content, with an increasing band width indicating successful integration of the kanamycin resistance gene into the target site. See the Experience tab for more details on the successful operation of this part in the MiamiU_OH project.

This part is only the coding region for kanamycin resistance, with the promoter BBa_K4047020 and terminator BBa_K4047021 being vital accessory components to its functional operation.

This part is an improvement to the documented part BBa_K1424003, which was optimized for Synechocystis sp. PCC 6803. This has been optimized here for use in the organism Synechococcus elongatus PCC 7942, and as such has a different DNA sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 782
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]