Difference between revisions of "Part:BBa K3861021"
 
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<partinfo>BBa_K3861021 short</partinfo>
 
<partinfo>BBa_K3861021 short</partinfo>
  
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This is the native promoter of the <i>Salmonella</i> typhimurium araBAD inducer system. P<sub>BAD</sub> initiates transcription during high L-arabinose and low glucose levels, as P<sub>BAD</sub> forms a complex with L-arabinose (Guzman et al., 1995).
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This is the native promoter of the <i>Salmonella</i> Typhimurium ara-operon. The ara-operon encodes genes for the breakdown of arabinose. Its expression is regulated by the AraC, which binds the P<sub>BAD</sub> in absence of arabinose. In the presence of arabinose, the AraC dimer inhibiting expression is released and the expression of downstream genes is allowed.<sup>1</sup>
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As a part of our project "TargetTaxi", we aim to use this promoter to regulate the expression of the SPI-1 master regulator, hilA. Thereby, allowing the overexpression of SPI-1 type 3 secretion machinery and its assembly at the cell poles. The presence of the machinery at the cell poles will result in its presence in the membrane of minicells.  
 
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K3861021 parameters</partinfo>
 
<partinfo>BBa_K3861021 parameters</partinfo>
 
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==='''References'''===
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1.    Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose P(BAD) promoter. J. Bacteriol. (1995) doi:10.1128/jb.177.14.4121-4130.1995.

Latest revision as of 19:08, 20 October 2021


araBAD promoter from Salmonella Typhimurium

This is the native promoter of the Salmonella Typhimurium ara-operon. The ara-operon encodes genes for the breakdown of arabinose. Its expression is regulated by the AraC, which binds the PBAD in absence of arabinose. In the presence of arabinose, the AraC dimer inhibiting expression is released and the expression of downstream genes is allowed.1
As a part of our project "TargetTaxi", we aim to use this promoter to regulate the expression of the SPI-1 master regulator, hilA. Thereby, allowing the overexpression of SPI-1 type 3 secretion machinery and its assembly at the cell poles. The presence of the machinery at the cell poles will result in its presence in the membrane of minicells. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Guzman, L. M., Belin, D., Carson, M. J. & Beckwith, J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose P(BAD) promoter. J. Bacteriol. (1995) doi:10.1128/jb.177.14.4121-4130.1995.