Difference between revisions of "Part:BBa K4047016"

Revision as of 18:39, 20 October 2021

Gentamicin resistance (GmR) for Synechococcus

Sequence used within the plasmid BBa_K4047039. This plasmid, which deletes a target gene by insertion of kanamycin. This part encodes gentamicin resistance (GmR) in the plasmid backbone. Successful transformants only integrate kanamycin for deletion of the target gene glpX. Incorrect crossover leads to integration of both kanamycin and gentamicin resistance genes, and does not prevent functional expression of glpX. Thus, the two genes in conjunction allow selection for glpX deletion. Although gentamicin resistance indicates failure of target gene deletion, it is extremely useful as a marker to differentiate single and double crossover transformants.

Some transformed colonies were able to grow on both kanamycin and gentamicin, indicating the successful expression and operation of this gene.

See the Experience tab for more details on the successful operation of this part in the MiamiU_OH project.

This part is only the coding region for gentamicin resistance, with the promoter BBa_K4047015 and terminator BBa_K4047017 being vital accessory components to its functional operation.

This part is optimized for operation in Synechococcus elongatus PCC 7942, exhibiting gentamicin resistance for low concentrations. This sequence varies from the established gentamicin resistance part BBa_P1014, which conveys resistance to gentamicin at up to 12 ug/ml in E. coli.

Sequence and Features

Long Description

Sequence encoding gentamicin resistance (gmr gene)