Difference between revisions of "Part:BBa K245114:Experience"

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[[File:T--NCTU_Formosa--bacteria_with_DenTeeth.png|500px|thumb|center|'''Figure 3. The growth curve of E. coli and P.gingivalis
 
[[File:T--NCTU_Formosa--bacteria_with_DenTeeth.png|500px|thumb|center|'''Figure 3. The growth curve of E. coli and P.gingivalis
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 Because P. gingivalis was in the RG2, so we could not do the LL-37 functional test by inhibit the growth of P. gingivalis. After reading some related papers, we found that the killing rate of E. coli was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test.
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[[File:T--NCTU_Formosa--bacteriatable.png|500px|thumb|center|'''Table 4. Parameters of the sterilization system of LL37
 
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Revision as of 18:17, 20 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K245114

User Reviews

UNIQc90a423d3deb7b4a-partinfo-00000000-QINU

•••••

ihua_li NCTU_Formosa(2021)

Colony Result

We successfully built DenTeeth with LL-37 .

Figure 1. Colony PCR result of DenTeeth after colony into E. coli BL21. (4685 b.p.)

Model

Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.

Figure 2. The growth curve of E. coli and P.gingivalis
Figure 3. The growth curve of E. coli and P.gingivalis

 Because P. gingivalis was in the RG2, so we could not do the LL-37 functional test by inhibit the growth of P. gingivalis. After reading some related papers, we found that the killing rate of E. coli was similar to that of P. gingivalis [4]. Based on these data, we determined to make E. coli, DH5α with pET32A, as the bacteria killed by DenTeeth in the LL-37 functional test.

Table 4. Parameters of the sterilization system of LL37
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BBa_K245114 NCTU-Formosa(2021) Not understood UNIQc90a423d3deb7b4a-partinfo-00000003-QINU