Difference between revisions of "Part:BBa K4047039:Experience"
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| − | This plasmid for | + | This plasmid is for deletion of SBPase, and was successfully transformed into Synechococcus elongatus cells. Using segregation PCR, we confirmed that only few copies of the genome could contain the inactivated SBPase with affecting cell viability, but the plasmid was able to successfully integrate the selective marker for kanamycin resistance into the target gene. |
===Applications of BBa_K4047039=== | ===Applications of BBa_K4047039=== | ||
Latest revision as of 17:58, 20 October 2021
This plasmid is for deletion of SBPase, and was successfully transformed into Synechococcus elongatus cells. Using segregation PCR, we confirmed that only few copies of the genome could contain the inactivated SBPase with affecting cell viability, but the plasmid was able to successfully integrate the selective marker for kanamycin resistance into the target gene.
Applications of BBa_K4047039
User Reviews
UNIQ76ab72c61c91edc0-partinfo-00000000-QINU UNIQ76ab72c61c91edc0-partinfo-00000001-QINU