Difference between revisions of "Part:BBa K3790014:Design"

Latest revision as of 17:57, 20 October 2021

The simplest form of dps promoter, a strong σS promoter

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The following primers were used for constructing this part.

F_dps_1035	5-ACACATAGCCGGTGCTATACTTAATCTCGACTAGAGAAAGAGGAGAAATACTAGATGC
R_dps_1907	5-GTATAGCACCGGCTATGTGTTCCGCTATTCCTCCCACTCCACCTGAACC

Source

The promoter sequence was isolated from E. coli genome.

References

[1] Gisela Becker, Regine Hengge-Aronis. (2001). What makes an Escherichia coli promoter σS dependent? Role of the 213/214 nucleotide promoter positions and region 2.5 of σS. Molecular Microbiology (2001) 39(5), 1153±1165.

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	===References===		===References===
		+	[1] Gisela Becker, Regine Hengge-Aronis. (2001). What makes an Escherichia coli promoter σS dependent? Role of the 213/214 nucleotide promoter positions and region 2.5 of σS. Molecular Microbiology (2001) 39(5), 1153±1165.