Difference between revisions of "Part:BBa K3790014:Design"
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===References=== | ===References=== | ||
+ | [1] Gisela Becker, Regine Hengge-Aronis. (2001). What makes an Escherichia coli promoter σS dependent? Role of the 213/214 nucleotide promoter positions and region 2.5 of σS. Molecular Microbiology (2001) 39(5), 1153±1165. |
Latest revision as of 17:57, 20 October 2021
The simplest form of dps promoter, a strong σS promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The following primers were used for constructing this part.
F_dps_1035 5-ACACATAGCCGGTGCTATACTTAATCTCGACTAGAGAAAGAGGAGAAATACTAGATGC R_dps_1907 5-GTATAGCACCGGCTATGTGTTCCGCTATTCCTCCCACTCCACCTGAACC
Source
The promoter sequence was isolated from E. coli genome.
References
[1] Gisela Becker, Regine Hengge-Aronis. (2001). What makes an Escherichia coli promoter σS dependent? Role of the 213/214 nucleotide promoter positions and region 2.5 of σS. Molecular Microbiology (2001) 39(5), 1153±1165.