Difference between revisions of "Part:BBa K3863005"

 
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<partinfo>BBa_K3863005 short</partinfo>
 
<partinfo>BBa_K3863005 short</partinfo>
  
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1. fabB B2323
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This is a composite part designed for the acid tolerant system.
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FabB: coding sequence for 3-oxoacyl-[acyl-carrier-protein] synthase from Escherichia coli (strain K12). (reference: NCBI locus tag - b2323)[1]   
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  1. Function:
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ldhA: Lactate dehydrogenase A (ldhA) is an enzyme catalyzing the synthesis of lactate from pyruvate. The protein sequence in this part is based on the K2588009 sequence Hamburg 2018.
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  Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor
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  Can also use unsaturated fatty acids
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  Catalyzes a key reaction in unsaturated fatty acid (UFA) synthesis, the elongation of the cis-3-decenoyl-ACP produced by FabA
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  Can use acyl chains from C-6 to C-14
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  1. Full name: 3-oxoacyl-[acyl-carrier-protein] synthase 1  
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1. ldhA (BBa_K2588009  IGEM 2018 iGEM18_Hamburg)
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  1. Function: Usually, under anaerobic conditions, E. coli may grow via two alternative energy-generating modes. If aspiration is not possible, because no electron-acceptors are present, bacteria start fermentation. Hereby, sugars are converted to reduced organic compounds for example lactic acid. The reaction is carried out via the lactate dehydrogenase which converts pyruvate into lactate under consumption of NADH.1 Therefore, ldhA can be easily used to produce lactic acid.
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  2. Full name: Lactate dehydrogenase
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<partinfo>BBa_K3863005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3863005 SequenceAndFeatures</partinfo>
  
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<h2>Reference</h2>
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<p>[1]https://www.ncbi.nlm.nih.gov/gene/946799</p>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 17:06, 20 October 2021


fabB-his-ldhA-flag

This is a composite part designed for the acid tolerant system. FabB: coding sequence for 3-oxoacyl-[acyl-carrier-protein] synthase from Escherichia coli (strain K12). (reference: NCBI locus tag - b2323)[1] ldhA: Lactate dehydrogenase A (ldhA) is an enzyme catalyzing the synthesis of lactate from pyruvate. The protein sequence in this part is based on the K2588009 sequence Hamburg 2018.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1240
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 991
    Illegal AgeI site found at 1841
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 571
    Illegal SapI.rc site found at 721
    Illegal SapI.rc site found at 1084
    Illegal SapI.rc site found at 1571

Reference

[1]https://www.ncbi.nlm.nih.gov/gene/946799