Difference between revisions of "Part:BBa K143075"
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− | AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP proein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>. | + | AmyE 3' Integration sequence(<bbpart>BBa_K143002</bbpart>) coupled to GFP proein coding region and the registry double terminator (part <bbpart>BBa_I13401</bbpart>). |
Revision as of 15:59, 20 May 2009
B. subtilis AmyE locus GFP output promoter and RBS tester
AmyE 3' Integration sequence(BBa_K143002) coupled to GFP proein coding region and the registry double terminator (part BBa_I13401).
The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.
The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644