Difference between revisions of "Part:BBa K3863004"
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<partinfo>BBa_K3863004 short</partinfo>
 
<partinfo>BBa_K3863004 short</partinfo>
  
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1. Phac (BBa_K2042001 from iGEM 2016 iGEM16_Evry)
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This is a composite part designed to produce bioplastic PHA with his-tag.
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PHA synthase 1 from Pseudomonas resinovorans. The protein sequence in this part is based on the K2042001 sequence Evry 2016.
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PCT is propionate CoA transferase from Clostridum propionicum. The protein sequence in this part is based on the K1211001 sequence Yale 2013. Tackling the issues with biobricks, we have optimized the codon.
  
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  1. Function: This is the coding sequence for class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer.
 
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  2. Full name: Pseudomonas resinovorans PHA synthase 1
 
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2. PCT (BBa_K1211001 from iGEM 2013 iGEM13_Yale)
 
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  1. Function: used this enzyme as the first enzyme in the pathway to create PLA or Polylactic acid. However, this biobrick produces (D)-Lactyl-CoA, which is the precursor to all PHAs
 
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  2. Full name: Clostridum propionicum propionate CoA transferase
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:54, 20 October 2021


Phac-flag-PCT(optimized)-HA-his

This is a composite part designed to produce bioplastic PHA with his-tag. PHA synthase 1 from Pseudomonas resinovorans. The protein sequence in this part is based on the K2042001 sequence Evry 2016. PCT is propionate CoA transferase from Clostridum propionicum. The protein sequence in this part is based on the K1211001 sequence Yale 2013. Tackling the issues with biobricks, we have optimized the codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
    Illegal BamHI site found at 1506
    Illegal XhoI site found at 1030
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 251