Difference between revisions of "Part:BBa K3989013"

Latest revision as of 16:46, 20 October 2021

double plant immune elicitors flg22 and elf18 fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display two plant immune elicitors, elf18 (Part:BBa_K3989023) and flg22 (Part:BBa_K3286138), to the surface of bacterial outer membrane vesicles (OMVs) to trigger plant immune responses. To make elf18 and flg22 better recognized by plant immune receptor FLS2, we put multiple linkers and a 3×FLAG tag between ClyA and two elicitors to minimize the steric hindrance.

Usage and Biology

This construct can be used to enhance the plant immune responses triggered by OMVs, please see: 2021 UZurich

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1204
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1204
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1204
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1204
Illegal NgoMIV site found at 1198
Illegal AgeI site found at 1162
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3989013 short</partinfo>
-Transmembrane protein Cly display epitopes flg22 and elf18
+In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display two plant immune elicitors, elf18 ([[Part:BBa_K3989023]]) and flg22 ([[Part:BBa_K3286138]]), to the surface of bacterial outer membrane vesicles (OMVs) to trigger plant immune responses. To make elf18 and flg22 better recognized by plant immune receptor FLS2, we put multiple linkers and a 3×FLAG tag between ClyA and two elicitors to minimize the steric hindrance.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<html>
+This construct can be used to enhance the plant immune responses triggered by OMVs, please see: <a href="https://2021.igem.org/Team:UZurich">2021 UZurich</a>
+</html>
 <!-- -->