Difference between revisions of "Part:BBa K3739054"

 
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===Biology===
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===Usage and Biology===
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Aly01 is a signal peptide from the alginate lyase of V.natriegens.
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PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue.
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his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.
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===Usage===
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By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.
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===Reference===
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1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.
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2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.
  
 
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Revision as of 16:46, 20 October 2021


J23100-B0030-Aly01-PPO-his-B0010

Aly01 is a signal peptide from the alginate lyase of V.natriegens and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion


Biology

Aly01 is a signal peptide from the alginate lyase of V.natriegens. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion.

Usage

By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739111.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.

Reference

1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.

2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]