Difference between revisions of "Part:BBa K4044001"

Revision as of 16:05, 20 October 2021

BphP1 (E.coli optimized)

BphP1 contain an N-terminal photosensory core domain (PCD) and a C-terminal effector domain that regulates the biological response. BphP1 has a phytochrome (PHY) motif with the chromophore biliverdin IXα (BV) covalently bound near the N-terminus. Most of the BphPs adopt inactive ground state with maximal absorption between 690 and 710 nm.

Canonical BphPs adopt an inactive ground (i.e., dark-adapted) conformation with maximal absorption between 690 and 710 nm. Upon exposure to red or short wavelength NIR light, these BphPs switch to the biologically active Pfr conformation, with maximal absorption between 750 and 760 nm. Canonical BphPs revert from Pfr to Pr in milliseconds after exposure to NIR light or in minutes to hours by thermal reversion in the dark. The bacteriophytochrome photoreceptor 1 (BphP1), found in several purple photosynthetic bacteria, senses NIR light and activates transcription of photosystem promoters by binding to and inhibiting the transcriptional repressor PpsR2.

We used truncated PpsR2 variant - QPas1 and fusing it to DNA binding domains of Gal4 from S. cerevisiae or LexA from Bacillus subtillis. DNA-binding domains not from E. coli and protein sensing NIR light opens a possibility for development of a fully orthogonal optogenetic system. We decided to change the sequence of BphP1 in presence of an already given variant (BBa_K3032016) as there was no data given if this protein could be effectively function in E.coli Саш остальное добавь про модели и оптимизацию кодонов

Sequence and Features